Macro and micro-structural study on Aspergillus parasiticus inoculated in peanut kernels treated with gamma radiation (Cs137)

Valéria Barbosa Borges, Maria Antonieta Peixoto Gimenes Couto, Mauro Carlos Lopes Souza, Áurea Maria Lages de Moraes, Marta Maria Braga Baptista Soares Xavier

Abstract


This study investigated the micro and macro-morphology of Aspergillus parasiticus, CMT 00064, inoculated into peanut kernels, after gamma irradiation with Cs137. Plates containing kernels inoculated with the fungal strain were incubated in a BOD germination chamber at 25oC for 5 days. These plates were irradiated at the dose rate of 1.6 kGy/min on the sixth day of incubation. The absorbed doses were: 0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0 kGy. The observed inactivation doses ranged from 4.5 to 8.0 kGy between days 1, 7 and 15 after irradiation. Fungus growth was observed 15 days after irradiation in the plates irradiated between 0 and 7.5 kGy demonstrating that the dose of 8.0 kGy could eliminate the fungus. Subcultures in nutrient media of isolates irradiated at 7.5 kGy recovered its growth in 15 days. Kernels irradiated with 6.5 kGy grew without metula indicating elimination. Colonies that grown after irradiation up 6.0 kGy were biseriate. However, after irradiation with 6.5 kGy they were uniseriate. The survival rates of A. parasiticus, in irradiated substrate peanuts, decreases with increasing absorbing doses of ionizing radiation.


Keywords


Peanuts, Aspergillus parasiticus, gamma irradiation, macro/micro-morphology

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References


ALVES, N. M. C.; ALMEIDA, F. A. C.; GOMES, J. P.; LEAL, A. S. C.; SILVA, M. M. Viability and mycroflora of irradiated seed of peanut with 60 cobalt (60Co), Revista Brasileira de Engenharia Agrícola e Ambiental, v. 15, p. 289-295, 2011.

BENTO, L. F.; CANEPPELE, M. A. B.; ALBUQUERQUE, M. C. F.; KOBAYASTI, L., CANEPPELE, C.; ANDRADE, P. J. Occurrence of fungi and aflatoxins in corn kernels, Revista do Instituto Adolfo Lutz, v. 71, p. 44-49, 2012.

BORGES, V. B.; MIRANDA, Z. B.; VITAL, H. C.; ROSA, C. A. R. Eliminação ou inativação de fungos do gênero Aspergillus em amendoim (Arachis hypogaea, L.) e o aumento de sua vida de prateleira, quando tratado com radiação gama. Higiene Alimentar, v. 21, p. 61-68, 2007.

BORGES, V. B.; MAIA, M. C. A.; COUTO, M. A. P. G.; HOLANDA, V. L.; SOUZA, M. C. L.; MORAIS, A. M. L. Alterações micro estruturais em cepas de referência de Aspergillus ochraceus induzidas por radiação gama em amendoim. Higiene Alimentar, v. 25, p. 1018-1019, 2011.

BORGES, V. B.; COUTO, M. A. P. G.; SOUZA, M. C. L.; MIRANDA, Z. B.; MORAIS, A. M. L. Estudo macroestrutural em cepas de referência de Aspergillus Flavus em grãos de amendoim irradiados. Higiene Alimentar, v. 26, p. 163-168, 2012.

BLANK, G.; CORRIGAN, D. Comparison of resistance of fungal spores to gamma and electron beam radiation, International Journal of Food Microbiology, v.26, p. 269-277. 1995.

BRACARENSE, A. A. P.; TAKAHASHI, J. A. Modulation of antimicrobial metabolites production by fungus Aspergillus parasiticus. Brasilian Journal of Mcirobiology, v. 45, p. 313-321, 2014.

COSTA, L. F.; SILVA, E. B.; OLIVEIRA, I. S. Irradiação gama em amendoim para controle de Aspergillus flavus, Scientia Plena, v. 9, p. 1-12, 2013.

DURAN, R. M.; CARY, J. W.; CALVO, A. M. Production of cyclopiazonic acid, aflatotrem and aflatoxin by Aspergillus flavus is regulated by veA, a gene necessary for sclerotial formation, Applied Microbiology and Biotechnology, v. 73, p. 1158-1168. 2007.

FACCA, M. C. L.; DALZOTO, P. R. Aflatoxinas: Um perfil da situação do amendoim e derivados no cenário brasileiro, Revista do Instituto Biológico, v. 72, p. 25-29, 2010.

FARKAS, J.; MOHÁCSI-FARKAS, C. History and future of food irradiation, Trends Food Science Technology, v. 22, p. 121-126, 2011.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS (FAO). Information on Post-Harvest Activities. 2010.

IMAMURA, K. B.; TONI, J. C. V.; BOCCHE, M. A. L.; SOUZA, D. A.; GIANNONI, J. A. Incidência de aflatoxinas no amendoim (Arachis hypogea L) cru em casca da região da Alta Paulista-SP, durante o período de 2011 e 2012, Revista do Instituto Adolfo Lutz, v. 73, p. 178-187, 2014.

INTERNATIONAL CONSULTIVE GROUP ON FOOD IRRADIATION (ICGFI). Code of good irradiation practice for the control of pathogenic microorganisms in poultry food – Report number 19, Vienna. 1995.

KLICH, M. A.; PITT, J. I. Differentiation of Aspergillus flavus from Aspergillus paraliticus and other closely related species, Transation of the British Mycological Society, Cambridge, London, v. 91, p. 99-108. 2002.

MAITY, J. P. Radiation-Induced effects on some common storage edible seeds India infested with surface microflora, Radiation Physics and Chemistry, v. 75, p. 1965-1072. 2004.

RIBEIRO, J. M. M.; VITAL, H. C.; MAGNOLI, C.; MERKIS, C.; CRISTOFOLILI, A.; ROSA, C. A. R. Alterações ultra estruturais em cepas de referência de Aspergillus spp. induzidas por irradiação gama, Revista Ciência e Vida, v. 28, p. 18-21, 2008.

RIBEIRO, J. M. M.; CAVAGLIERI, I. R.; CRISTOFOLINI, A.; ROSA, C. A. R.; VITAL, H. C.; MERKIS, C.; ASTORECA, A.; ORLANDO, J.; CARU, M.; DALCERO A. Effect of gamma irradiation on Aspergillus flavus and Aspergillus ochraceus ultrastructure and mycotoxin production, Radiation Physics and Chemistry, v. 80, p. 658-663, 2011.

ROSSETTO, C. A. V.; VIEGAS, E. C.; LIMA, T. M. Contaminação fúngica do amendoim em função das doses de calcário e das épocas de amostragem, Bragantia, v. 62, p. 437-445, 2003.

RUDREW, S.; CRAFT, J.; AIDOO, K. Occurrence of toxigenic Aspergillus spp. And aflatoxins in selected food commodities of Asian origin in the west of Scotland. Food and Chemical Toxicology, v. 55, p. 653-658, 2013.

SANTOS, F.; MEDINA, P. F.; LOURENÇÃO, A. L.; PARISI, J. J. D.; GODOY, I. J. Qualidade de sementes de amendoim armazenadas no estado de S. Paulo, Bragantia, v. 72, p. 310-317, 2013.

SANTOS, T. S.; ALMEIDA, F. A. C.; SUASSUNA, T. M. F.; COUTINHO, W. M.; ALMEIDA, P. B. A. Resposta de sementes de amendoim a diferentes doses de radiação gama do cobalto 60 (60Co)

Revista Brasileira de Engenharia Agrícola e Ambiental, v. 14, p. 1074-1078, 2010.

STOEV, S. D. Food safety and increasing hazard of mycotoxin occurrence in food and seeds, Critical Reviews in Food Science and Nutrition, v. 53, p. 887-901, 2013.

WANG, F.; XIE, F.; XUE, X. F.; WANG, Z.; FAN, B.; HA, Y. M. Structure elucidation and Toxicity analyses of the radiolytic products of aflatoxin B1 methanol-water solution, Journal of Hazard Mater, v. 192, p. 1192-1203, 2011.

ZORZETE, P.; REIS, T. A.; FELICIO, J. D.; BAQUIÃO, A. C.; MAKIMONTO, P.; CORREA B. Fungi, mycotoxins and phytoalexin in peanut varieties during plant growth in the field, Food Chemistry, v. 129, p. 957-964, 2011.

ZORZETE, P.; ATAYDE, D. D.; REIS, T. A.; GONÇALEZ, E.; BAQUIÃO, A. C.; CORREA, B. Microbiota, aflatoxins and cyclopiazonic acid in stored peanut cultivars, Food Research International, v. 52, p. 380-386, 2013.




DOI: http://dx.doi.org/10.18067/jbfs.v4i1.127

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