Protein content in Glycine max grains influenced by the mixed inoculation of Bradyrhizobium japonicum and Azospirillum brasilense

Daisy Parente Dourado, Joenes Mucci Peluzio, Evandro Reina, Kayo Kennedy Albernas, Thiago Magalhães de Lázari, Cid Tacaoca Muraishi

Abstract


Soy is mainly valued for high protein content when compared to other crops. However, it had been common to visit genetic materials with the same lower and lower protein levels, a fact that mainly displeased animal feed manufacturers. The objective of this study was to evaluate Azospirillum and Bradyrhizobium on the protein content of soybeans, grown under low latitude. The experimental design was a randomized complete block design with two cultivars (TMG 1288 RR and M 9144 RR) and five doses of Azospirillum brasilense (0, 100, 200, 300 and 400 mL ha-1). The evaluation was performed at the R8 stage, where the heavy, weighted and measured variables and the protein content of the grains (%) were analyzed. The mixed use of Bradyrhizobium via seed and Azospirillum via foliar caused an increase in protein levels, in all planting seasons and cultivars. High and precipitated low temperatures contributed to the increase of grain protein contents, when in the presence of Azospirillum, excess of cultures by environmental parameters and genetic factors, but also for the nutritional factors that are metabolized by the plant and assimilated in the form of reserves without grain, due to the application of associative bacteria.


Keywords


Inoculant; Protein increase; Phytohormones; Water restriction; Soybean

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References


Albrecht, L. P., Braccini, A. L., Ávila, M. R., Suzuki, L. S., Scapim, C. A., & Barbosa, M. C. (2008). Teores de óleo, proteínas e produtividade de soja em função da antecipação da semeadura na região oeste do Paraná. Bragantia, 67(4), 865-873. https://doi.org/10.1590/S0006-87052008000400008

Bewley, J. D., & Black, M. (1985). Seeds: Physiology of Development and germination. New York: Plenum Press, 367p. https://doi.org/10.1007/978-1-4615-1747-4

Campos, L. J. M., Costa, R. V., Almeida, R. E. M., & Simon, J. (2016). Desempenho de cultivares de soja na safra 2015/2016. Informativo Técnico: Fronteira Agrícola nº10. Núcleo de Sistemas Agrícolas da Embrapa Pesca e Aquicultura, 2016.

Cavatte, P. C., Martins, S. C. V., Morais, L. E., Silva, P. E. M., Souza, L. T., & Matta, F.M. da. A fisiologia dos estresses abióticos. In: FRITSCHE-NETO, R., BORÉM, A. Melhoramento de plantas para condições de estresses abióticos. Visconde do Rio Branco: Suprema, 2011. cap.3, p.39-47. PMid:21939445

Cerezini, P., Kuwano, B., Santos, M., Terassi, F., Hungria, M., & Nogueira, M. A. (2016). Strategies to promote early nodulation in soybean under drought. Field Crops Research, 196(1), p.160-167, 2016. https://doi.org/10.1016/j.fcr.2016.06.017

Cohen, A. C., Bottini, R., & Piccoli,P. N. (2008). Azospirillum brasilense Sp 245 produces ABA in chemically-defined culture medium and increases ABA content in arabidopsis plants. Plant Growth Regulation, 54(3), 97-103, 2008. https://doi.org/10.1007/s10725-007-9232-9

Conab – Companhia Nacional de Abastecimento. Monitoramento agrícola (2016). Acompanhamento da safra brasileira Grãos, v. 4 Safra 2016/17-Oitavo levantamento, Brasília, maio 2017. Recuperado de http://www.conab.gov.br/OlalaCMS/uploads/arquivos/17_05_12_10_37_57_boletim_graos_maio_2017.pdf

Cruz, C. D., Regazzi, A. J., & Carneiro, P. C. S. (2014). Modelos biométricos aplicados ao melhoramento genético. Viçosa: UFV, 514 p.

Dodd I. C., Zinovkina N. Y., Safronova V. I., & Belimov A. A. (2010). Rhizobacterial mediation of plant hormone status. Annals of Applied Biology, 157(3), 361-379. https://doi.org/10.1111/j.1744-7348.2010.00439.x

Embrapa - Empresa Brasileira de Pesquisa Agropecuária (2013). Sistema brasileiro de classificação de solos. Brasília: Embrapa Solos, 353 p.

Fehr, W. R., Caviness, R. E., Burmood, D. T., Pennineton, J. S. (1971). Stage of development descriptions for soybeans, Glycine max L. Merrill. Crop Science, 11(6), 929-931. https://doi.org/10.2135/cropsci1971.0011183X001100060051x

Ferreira, D. F. (2011). Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, 35(6), 1039-1042. https://doi.org/10.1590/S1413-70542011000600001

Freiria, G. H., Lima, W. F., Leite, R. S., Mandarino, J. M. G., Silva, J. B., & Prete, C. E. C. (2016). Productivity and chemical composition of food-type soybeans sown on different dates. Acta Scientiarum, Agronomy, 38(3), 371-377. https://doi.org/10.4025/actasciagron.v38i3.28632

Galindo, F. S., Teixeira Filho, M. C. M., Buzetti, S., Santini, J. M. K., Alves, C. J., Nogueira, L. M., Ludkiewicz, M. G. Z., Andreotti, M., & Bellotte, J. L. M. (2016). Corn Yield and Foliar Diagnosis Affected by Nitrogen Fertilization and Inoculation with Azospirillum brasilense. Revista Brasileira de Ciência do Solo, 40(1), 1-18. https://doi.org/10.1590/18069657rbcs20150364

Hungria, M., Nogueira, M.A., & Araujo, R.S. (2013). Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability. Biology and Fertility of Soils, 49(7), 791-801. https://doi.org/10.1007/s00374-012-0771-5

Hungria, M., Nogueira, M. A., & ARAUJO, R. S. (2015). Soybean Seed Co-Inoculation with Bradyrhizobium spp. and Azospirillum brasilense: A new biotechnological tool to improve yield and sustainability. American Journal of Plant Sciences, 6(6), 811-817. https://doi.org/10.4236/ajps.2015.66087

Kaschuk, G., Leffelaar, P. A., Giller, K. E., Alberton, O., Hungria, M., & Kuyper, T. W. (2010a). Responses of grain legumes to rhizobia and arbuscular mycorrhizal fungi: a meta-analysis of potential photosynthate limitation of symbioses. Soil Biology & Biochemistry, 42(1), 125-127. https://doi.org/10.1016/j.soilbio.2009.10.017

Kaschuk, G., Hungria, M., Leffelaar, P. A., Giller, K. E., & Kuyper, T. W. (2010b). Differences in photosynthetic behaviour and leaf senescence of soybean (Glycine max [L.] Merrill) dependent on N2 fixation or nitrate supply. Plant Biology, 12(1), 60-69. https://doi.org/10.1111/j.1438-8677.2009.00211.x

Kaschuk, G., Horst, R., Santos, M. A., Colauto, G. A. L., & Alberton, O. (2013). Relações prováveis entre nodulação, parte aérea e clorofila de variedades de soja com diferentes teores de proteína e óleo nos grãos. Journal of Agronomic Sciences, 2(1),170-178, 2013.

Lopes, J. A. M., Peluzio, J. M., Martins, & G. S. (2016). Teor de proteína e óleo em grãos de soja, em diferentes épocas de plantio para fins industriais. Tecnologia & Ciência Agropecuária.10(3), 49-53.

Marcos Filho, J. (2005). Fisiologia de sementes de plantas cultivadas. (5a ed). Piracicaba: FEALQ, 495p.

Nascimento, R., & Mosquim, P. R. (2004). Crescimento e teor de proteínas em sementes de soja sob influência de hormônios vegetais. Revista Brasileira de Botânica, 27(3), 573-579.

Pinheiro, L. C. M., God, P. I. V. G., Faria, V. R., Oliveira, A. G., Hasui, A. A., Pinto, E. H. G., Arruda, K. M. A., Piovesan, N. D., & Moreira, M. A. (2013). Parentesco na seleção para produtividade e teores de óleo e proteína em soja via modelos mistos. Pesquisa Agropecuária Brasileira, 48(9), 1246-1253. https://doi.org/10.1590/S0100-204X2013000900008

Portugal, J. R., Arf, O., Peres, A. R., Gitti, D. C., Rodrigues, R. A. F. Garcia, N. F. S., & Garé, L. M. Azospirillum brasilense promotes increment in corn production. African Journal of Agricultural Research. 11(19), 1688-1698. https://doi.org/10.5897/AJAR2015.10723

Sales, P. V. G., Peluzio, J. M., Aferri, F.S., Sales, A. C. R., & Sales, V. H. G. (2016). Effect of pods' position on the protein content in soybean grains at low latitude. Journal of Bioenergy and Food Science, 3(4), 216-221. https://doi.org/10.18067/jbfs.v3i4.102

Saubidet, M. I., Fatta, N., Barneix, A. J. (2002). The effect of inoculation with Azospirillum brasilense on growth and nitrogen utilization by wheat plants. Plant and Soil, 245(1), 215-222. https://doi.org/10.1023/A:1020469603941

Taiz, L. & Zeiger, L. 2002. Plant Physiology. 3rd ed. Sinauer Associates, Sunderland.

PMCid:PMC152206

Huizen, R. van, Ozga, J. A., & Reinecke, D. M. (1996). Influence of auxin and gibberellin on in vivo protein synthesis during early pea fruit growth. Plant Physiology, 112(1), p.53-59, Recuperado de https://www.ncbi.nlm.nih.gov/pmc/articles/PMC157922/pdf/1120053.pdf

Zuffo, A. M., Rezende, P. M., Bruzi, A. T., Oliveira, N. T., Soares, I. O., Neto, G. F. G., Cardillo, B. E. S., & Silva, L. O. (2015). Co-inoculation of Bradyrhizobium japonicum and Azospirillum brasilense in the soybean crop. Revista Ciências Agrárias, 38(1), 87-93.




DOI: http://dx.doi.org/10.18067/jbfs.v5i2.146

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