GRAS substances in control of Colletotrichum gloeosporioides growth and Fusarium guttiforme vitro / Substâncias GRAS no controle do crescimento de Colletotrichum gloeosporioides e Fusarium guttiforme in vitro.

Eskálath Morganna Silva Ferreira, Camilla Martins Malta, Cristiane Martins Coelho, Raphael Sanzio Pimenta


Colletotrichum gloeosporioides and Fusarium guttiforme are phytopathogenic fungi causing various post-harvest diseases in fruits. Substances "GRAS" - (Generally Regarded Ase Safe), they are an alternative for the control of microorganisms in the post-harvest phase, due to their fungistatic and fungicide effects. The objective of this study was to evaluate the inhibitory effects of five "GRAS" substances over the growth of pathogenic fungi C. gloeosporioides and F. guttiforme in vitro. Culture media (PDA) were supplemented with 1%, 3% and 5% sodium bicarbonate, sodium carbonate, calcium carbonate, calcium chloride and 0.1% 0.5% 1% potassium chloride. 10?l spore solution was inoculated at a concentration of 5x103ml/spore. From the five substances used in the tests, sodium bicarbonate and sodium carbonate inhibited 100% of C. gloeosporioides growth, the others were not limiting to the development of the fungus when compared to control. Sodium carbonate inhibited 100% of F. guttiforme growth at all concentrations. Were also positive, sodium bicarbonate in concentrations of 3% and 5%. The treatment with 5% calcium chloride at 51% reduced the growth this phytopatogen. Other substances not reduced or inhibited the mycelial growth of the pathogen when compared to the control. From the five substances "GRAS" tested in this work, sodium carbonate and sodium bicarbonate, were effective in inhibiting the mycelial growth of C. gloeosporioides. Sodium carbonate, sodium bicarbonate and calcium chloride were able in to reduce the growth of F. guttiforme


Colletotrichum gloeosporioides, Fusarium guttiforme, integrated biological control; Biocontrol; Sodium bicarbonate


AGRIOS, G. N. Plant pathology. 5 th ed. San Diego: Academic Press, 2005. 922p.

BAUTISTA-BAÑOS, S.B.; SIVAKUMAR,D.; BELLO-PERÉZ, A.; VILLANUEVA, A.; HERNADEZ-LOPEZ,M. A review of the management alternatives for controlling fungi onpapaya fruit during the postharvest supply chain. Crop Protecion.v.49.2013.8-20p.

BONETT, L.P.; MULLER, G.M.; WESSLING, C.R.; GAMELLO, F.D. Extrato etanólico de representantes de cinco famílias de plantas e óleo essencial da família Asteraceae sobre o fungo Colletotrichum gloeosporioides coletados de frutos de mamoeiro (Carica papaya L.). Rev. Bras. de Agroecologia. 7, p.116-125, 2012.

CANNON, P.F.; DAMM, V.; JOHNSTON, P.R., WEIR, B.S. Colletotrichum-current status and future directions. Stud Mycol. 2012.73. 181-213p.

GUPTA. V. K. et al. Genetic characterization of mango anthracnose pathogen Colletotrichum gloeosporioides Penz. By random amplified polymorphic DNA analysis. African Journal of Biotechnology, v. 9, n. 26, p. 4009-4013, 2010.

HILLOCKS, R.J. Farming with fewer pesticides: EU pesticide review and resulting challengesfor UK agriculture. Crop Protection, v. 31, p.85 e 93, 2012.

JANISIEWICZ, W. J; KURTZMAN, C. P.; BUYER, J. S. Yeasts associated with nectarines and their potential for biological control of brown rot. Yeast. v.27, p. 389-398, 2010.

JANISIEWICZ, J.W.; CONWAY, W.S. Combining biological control with physical and chemical treatments to control fruit decay after harvest. Stewart Postharvest Review, P.1- 3, 2010.

KUPPER, K.C.; CERVANTES, A.L.L.; KLEIN,M.N.; SILVA, A.C. Avaliação De Microrganismos Antagônicos, Saccharomyces cerevisiae e Bacillus subtilis para o controle de Penicillium digitatum. Rev. Bras. Frutic., Jaboticabal - SP, v. 35, n. 2, p. 425-436,2013.

MADANI, B.; MOHAMED, M.T.M.; BIGGS, A.R.; KADIR, J.; AWANG,Y.; TAYEBIMEIGOONI, A.; SHOJAEI, T.R. Effect of pre-harvest calcium chloride applications on fruit calciumlevel and post-harvest anthracnose disease of papaya. Crop Protection.v.55, p.55-60, 2013.

MELO, I.S.; AZEVEDO, I.L. Controle Biológico. Jaguariuna: Embrapa Meio Ambiente. v.3, 387p, 2000.

TALIBI, H.; BOUBAKER, H.; BOUDYACH, E.H ; BEN AOUMAR, A.A. Alternative methods for the control of postharvest citru. Journal of Applied Microbiology, P.3-17, 2014.

PIMENTA, R.S.; MORAIS, P.B.; ROSA, C.A.; CORRÊA, J. Utilization of Yeasts in Biological Control Programs, Yeast Biotechnology: Diversity and Applications. Springer Science, 200-212P., 2009.

PIMENTA, R.S.:, SILVA, J.F.M.; BUYER, J.S.; JANISIEWICZ, W.J. Endophytic Fungi from Plums (Prunus domestica) and Their Antifungal Activity against Monilinia fructicola. Journal of Food Protection, Vol. 75, No. 10, Pages 1883–1889, 2012.

RAMPERSAD, S. N. Molecular and phenotypic characterization of colletotrichum species associated with Antracnose desiase of papaya in Trinidade. Plant dis. 95. 1244-1254. 2011.

ST?PIE?, L.; KOCZYK, G.; WA?KIEWICZ, A. Diversity of Fusarium species and mycotoxins contaminating pineapple. J Appl Genetics v.5, p.367–380, 2013



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