Evaluation of antagonistic activity in vitro of endophytic fungi associated with camu-camu (Myrciaria dubia) / Avaliação da atividade antagonista in vitro de fungos endofíticos associados ao camu-camu (Myrciaria dubia)

Vanessa Carolina de Sena Correia, Rita de Kássia de Oliveira Pereira, Nathália Oliveira Lima, Maildo Barbosa Coelho, Waldesse Piragé de Oliveira Júnior, Raphael Sanzio Pimenta


Endophytic fungi are those that inhabit the inner tissue of their hosts, playing varied and narrow ecological relations without showing visible symptoms. It is estimated that the majority of plant species have not yet been sorted endophytic microorganisms and little-known properties, but some potentially suitable for biotechnological applications. Camu-camu (Myrciaria dubia) has great economic potential, the nutritional characteristics of the fruit, such as high production of vitamin C. Were isolated and cultured 307 endophytic fungi associated with the leaves and stems of M. dubia in order to verify the antagonistic activity, for the production of diffusible and volatile substances against the following pathogens: M. fructicola, Colletotrichum gloeosporioides and Aspergillus parasiticus. It was found that 22 isolates inhibited the growth of at least one target plant pathogens, showing inhibition potential (IP) from 25.62% to 93.84%, five endophytic stood with inhibition percentage above 50%, by producing diffusible and volatile substances. The competitive interactions were analyzed according to Badalyan scale, and there was a higher frequency among the fungi, the interaction type A ("deadlock" with mycelial contact). It is concluded that endophytic fungi isolated from leaves and stems of M. dubia, have promising biotechnological potential in biocontrol of plant pathogens (A.parasiticus, M. fructicola and C. gloeosporioides).


Paired culture, Plant pathogens, Diffusible, Volatile, Myrciaria dubia


ALMEIDA, T. F.; PEREIRA, C. F.; CAMARGO, M.; PANIZZI, R. C. Efeito do óleo de nim e extratos vegetais de Azadirachia indica, Cymbopogon citratus e Zingiber officinale no crescimento micelial in vitro de Diaporthe citri. Summa Phytopathologica 30:116, 2004.

BADALYAN, S. M.; INNOCENTI, G.; GARIBYAN, N. G. Antagonistic activity of xylotrophic mushrooms against pathogenic fungi of cereals in dual culture. Phytopathol Mediterr , v. 41, p.200–225, 2002.

BENÍTEZ, T.; RICÓN, A. M.; LIMÓN, M. C.; CODÓN, A. C.; Biocontrol mechanisms of Trichoderma strains. International Microbiology, v.7, 249-260, 2004.

BOMFIM, M. P.; SÃO JOSÉ, A. R.; REBOUÇAS, T. N. H.; ALMEIDA, S. S. D.; SOUZA, I. V. B.; DIAS, N. O. Avaliação antagônica in vitro e in vivo de Trichoderma spp. a Rhizopus stolonifer em maracujazeiro amarelo. Summa Phytopathologica, 2010.

CAMPANILE, G.; RUSCELLI, A.; LUISI, N.; Antagonistc activity of endophytic fungi towards Diplodia corticola assessed by in vitro and in planta test. European Journal of Plant Pathology, v.117: 237-246, 2007.

CORABI-ADELL, C. Biodiversidade do gênero Trichoderma (HYPOCREALES - FUNGI) mediante técnicas moleculares e análise ecofisiográfica. 220 f. 2004 Tese de Doutorado, Instituto de Biociências, Universidade Estadual de São Paulo, Rio Claro, 2004.

EDGINTON, L. V.; KNEW, K. L.; BARRON, G. L. Fungitoxic spectrum of benzimidazole compounds. Phytopathology, Saint Paul, v. 62, n. 7, p. 42 - 44, 1971.

GRIMME, E.; ZIDACK, N. K.; SIKORA, R. A.; STROBEL, G. A.; JACOBSEN, B. J. Comparison of Muscodor albus volatiles with a biorational mixture for control of seedling diseases of sugar beet and root-knot nematode on tomato. Plant Disease, v.91, p.220-225, 2007.

HOWELL, C. R. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis, v. 87, 4–10, 2003.

KUNOH, H. Endophytic actinomycetes: attractive biocontrol agents. Journal of General Plant Pathology, v.68, p.249-252. 2002.

KUPPER, K. C.; FERNANDES, N. G.; GOES, A. Controle biológico de Colletotrichum acutatum, agente causal da queda prematura dos frutos cítricos. Fitopatologia Brasileira, Brasília, v. 28, n. 3, p. 251 - 257, 2003.

LEELASUPHAKUL, W.; HEMMANEE, P.; CHUENCHITT, S. Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit. Postharvest Biology and Technology, v. 48, n.1, 113–121, 2008.

LIMA, L. H. C.; DE MARCO, J. L.; FELIX, C. R. Enzimas hidrolíticas envolvidas no controle biológico por micoparasitismo. In: Controle Biológico, v. 2, p. 263-304, 2000.

LIU, L. N.; ZHANG, J. Z.; XU, T. Histopathological studies of sclerotia of Rhizoctonia solani parasitized by the EGFP transformant of Trichoderma virens. Letters in Applied Microbiology, v.49, p.745-750, 2009.

LOUZADA, G. A.; CARVALHO, D. D. C.; MELLO, S. C. M.; JÚNIOR, M. L.; MARTINS, I.; BRAÚNA, L. M. Potencial antagônico de Trichoderma spp. originários de diferentes agroecossistemas contra Sclerotinia sclerotiorum e Fusarium solani. Biota neotropica, v. 9, n. 3, 145-149, 2009.

MARTINS, I.; PEIXOTO, J. R.; MENÊZES, J. E.; MELLO, S. C. M. Avaliação in vitro do antagonismo de Trichoderma spp. sobre Colletotrichum gloeosporioides. Boletim de Pesquisa e Desenvolvimento (INFOTECA-E), p.12, 2007.

MOREIRA, L. M.; MAY-DE MIO, L. L. Efeito de fungos antagonistas e produtos químicos no controle da podridão parda em pomares de pessegueiro. Floresta, v. 36, n. 2, p. 287-293, 2006.

MOREIRA, L. M.; MAY-DE MIO, L. L. Controle da podridão parda do pessegueiro com fungicidas e fosfitos avaliados em pré e pós-colheita. Ciênc. Agrotec., v. 33, n. 2, p. 405-411, 2009.

ORLANDELLI, R. C.; DE ALMEIDA, T. T.; ALBERTO, R. N.; POLONIO, J. C.; AZEVEDO, J. L.; PAMPHILE, J. A. Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw. Brazilian Journal of Microbiology. v.46, n. 2, 359-366, 2015.

PERAZA-SANCHEZ, S. R.; CHAN-CHE, E. O.; RUIZ-SANCHEZ E. Screening of Yucatecan plant extracts to control Colletotrichum gloeosporioides and isolation of a new pimarene from Acacia pennatula. J Agric Food Chem. v. 53, p.2429–2432, 2005.

PIMENTA, R. S.; SILVA, F. L.; SILVA, J. F.; MORAIS, P. B.; BRAGA, D. T.; ROSA, C. A.; CORRÊA Jr, A. Biological control of Penicillium italicum, P. digitatum and P. expansum by the predacious yeast Saccharomycopsis schoenii on oranges. Brazilian Journal of Microbiology, v. 39, n. 1, 85-90, 2008.

PIMENTA, R. S.; DA SILVA, J. F. M.; BUYER, J. S.; JANISIEWICZ, W. J. Endophytic fungi from plums (Prunus domestica) and their antifungal activity against Monilinia fructicola. Journal of Food Protection, 75(10), 1883-1889, 2012.

POLLI, A.; NEVES, A. F.; GALO, F. R.; GAZARINI J.; RHODEN, S. A.; PAMPHILE J. A. Aspectos da interação dos micro-organismos endofíticos com plantas hospedeiras e sua aplicação no controle biológico de pragas na agricultura. SaBios: Rev. Saúde e Biol., v.7, n.2, p.82-89, 2012.

PUNJA, Z. K.; UTKHEDE, R. S. Using fungi and yeasts to manage vegetable crop diseases. Trends in Biotechnology, v. 21, n. 9, p. 400-407, 2003.

RAMOS, D. M. B.; SILVA, C. F.; BATISTA, L. R.; SCHWAN, R. F. Inibição in vitro de fungos toxigênicos por Pichia e Debaryomyces sp isoladas de frutos de café (Coffea arabica). Acta Sci Agron, v.32, 397–402, 2010.

RUBINI, M. R.; SILVA-RIBEIRO, R. T.; POMELLA, A. W. V.; MAKI, C. S.; ARAÚJO, W. L.; SANTOS, D. R.; AZEVEDO, J. L. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of Witches’ Broom Disease. International Journal of Biological Sciences, Bethesda, v. 1, p. 24-33, 2005.

SCHULZ, B.; BOYLE, C. The endophytic continuum. Mycological Research, Cambridge, v. 109, p. 661-686, 2005.

STONE, J. K.; BACON, C. W.; WHITE, J. F. An overview of endophytic microbes: endophytism defined. In: BACON, C.W. & WHITE, J.F. (Eds.). Microbial Endophytes. New York: Marcel Dekker, p. 3-30, 2000.

STROBEL, G. A. Endophytes as sources of bioactive products. Microbes and Infection, v.5, p.535-544, 2003.

SUTO, M.; TAKEBAYASHI, M.; SAITO, K.; TANAKA, M.; YOKOTA, A.; TOMITA, F. Endophytes as producers of xylanase. Journal of Bioscience and Bioengineering, v. 93, n. 1, p. 88-90, 2002.

TARKKA, M. T.; PIECHULLA, B. Aromatic weapons: truffles attack plants by the production of volatiles. New Phytologist, v. 175, 383–386, 2007.

VEY, A.; HOAGLAND, R. E.; BUTT, T. M. Toxic metabolites of fungal biocontrol agents. In: BUTT, T.M.; JACKSON, C.N. Fungi as biocontrol agents: progress, problems and potential. CAB International, p.311-46, 2001.

WENZEL, J. B. ISOLAMENTO E ATIVIDADE ANTAGONÍSTICA DE FUNGOS ENDOFÍTICOS DE SOJA (Glycine max (L.) Merrill). SaBios-Revista de Saúde e Biologia, v. 7, n. 3, 2012.

WHEATLEY, R. E. The consequences of volatile organic compound mediated bacterial and fungal interactions. Antonie Van Leeuwenhoek, Amsterdam, v.81, p.357-364, 2002.

WOODS, C. M.; WOODWARD, S.; REDFERN, D. B. In vitro interactions in artificial and wood-based media between fungi colonizing stumps of Sitka spruce. Forest Pathology, Berlin, v. 35, p. 213-229, 2005.

XIAO-YAN, S.; QING-TAO, S.; SHU-TAO, X.; XIU-LAN, C.; CAI-YUN, S.; ZHANG ZHONG, Y. Broad-spectrum antimicrobial activity and high stability of Trichokonins from Trichoderma koningii SMF2 against plant pathogens. FEMS Microbiology Letters, v. 260, n. 1, p. 119–125, 2006.

ZUCCHI, T. D.; DE MELO, I. S. Controle biológico de fungos aflatoxigênicos. Biocontrole de Doenças de Plantas: Uso e Perspectivas. Jaguariúna-SP: Embrapa, p. 69-74, 2009.

DOI: http://dx.doi.org/10.18067/jbfs.v2i4.74


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