Production of extracellular enzymes by fungi associated to the decomposition of plant materials in streams / Produção de enzimas extracelulares por fungos associados à decomposição materiais vegetais em riachos

Jéssica Barros Aguiar Silva, Suélen Caroline Frantz, Anelise Kappes Marques, Cristiane Martins Coelho, Paula Benevides de Morais

Abstract


Fungal cellulases and xylanases derived from the huge degradative capabilities of these microorganisms and excreted to medium, raise a biotechnologixcal interest due to large application potential. The objective of this paper is to identify the production of the enzymes cellulase and xylanase by filamentous fungi isolated from allochthonous leaf litter in a stream in Cerrado ecosystem. The tests were carried out using 100 strains grown in synthetic culture medium containing carboxymethyl cellulose or beechwood xylan as sole carbon source for cellulase and xylanase production detection respectively. Inocula were performed in the center of plates and stored for 4 days at 28 ° C and subjected afterwards to thermal shock at 50 ° C for 16 hours. Plates were stained with Congo red solution and washed with a NaCl solution for betterdetection of the hydrolysis halo. Sixty nine percent of the fungal strains tested were positive for cellulase of which 23% were considered with potential for application in biotechnology. None of the fungal strains was able to degrade xylan


Keywords


celulase, xilanase, filamentous fungi

References


ABDEL-RAHEEM, A.; SHEARER, C. A. Extracellular enzyme production by freshwater ascomycetes. Fungal Diversity, v. 11, p. 1-19, 2002.

BEGON, M.; TOWNSEND, C. R.; HARPER, J. L. Ecologia: de indivíduos a ecossistemas. Artmed: Porto Alegre. 2007. 752p

CASTELLANI, S.Viability of Some Pathogenic Fungi in Distilled Water. Journal of Tropical Medicine and Hygiene, v. 42, p. 225-226,1939.

COLEN, G.; JUNQUEIRA, R. G.; SANTOS, T. M. Isolation and screening of alkaline lipase-producing fungi from Brazilian savanna oil. World Journal of Microbiology and Biotechnology, v. 22, p. 881-885, 2006.

DASHTBAN, M.; SCHRAFT, H.; QIN, W. Fungal bioconversion of lignocellulosic residues; opportunities and perspectives. International Journal of Biological Sciences, v. 5, n. 6, p. 578–595, 2009.

FAHEINA Jr., G.; BRAGA, R. M.; LOPES, V. R.; MARTINS, C.; PINTO, G. Atividade xilanolítica em cepas de Fusarium. In: XVII SIMPÓSIO NACIONAL DE BIOPROCESSOS. 2009. Natal – RN.

FARINAS, C. S.; LEMO, V.; RODRÍGUEZ-ZÚÑIGA, U. F.; BERTUCCI, N. V.; COURI, S. Avaliação de diferentes resíduos agroindustriais como substratos para a produção de celulases por fermentação semi-sólida. Boletim Nacional de Desenvolvimento. Embrapa: São Carlos. 2008. 15 p.

GONÇALVES JR., J. F.; CALLISTO, M. Organic-matter dynamics in the riparian zone of a tropical headwater stream in Southern Brasil. Aquatic Botanic, v. 109, p. 8-13, 2013.

GONÇALVES JR, J. F.; GRAÇA, M. A. S.; CALLISTO, M. Litter decomposition in a Cerrado savannah stream is retarded by leaf toughness, low dissolved nutrients and a low density of shredders. Freshwater Biology, v.52, p. 1440-1451, 2007.

HRMOVA, M.; BEILY, P.; VRSANKA, M. Cellulose and xylan degrading enzymes by Aspergillus terreus. Enz. Microb. Tech., v. 11, p. 610-616, 1989.

HRMOVA, M.; PETRAKOVA, E.; BIELY, P. Induction of cellulose and xylandegrading enzyme systems in Aspergillus terreus by homo and hetero-disaccharides composed of glucose and xylose. J. Gen. Microbiol., v.137, p.541-547, 1991.

KAR, S.; MANDAL, A.; MOHAPATRA, P. K.; MONDAL, K. C., PATI, B. R. Production of cellulase-free xylanase by Trichoderma reesei SAF3. Braz J Microbiol, v. 37, n. 4, p. 462-464, 2006.

KASANA, R. C.; SALWAN, R.; DHAR, H.; DUTT, S.; GULATI, A. A rapid and easy method for the detection of microbial cellulases on agar plates using gram’s iodine. Curr Microbiol, v. 57, p. 503–507, 2008.

KURTBÖKE, D.I., SWINGS, J. & STORMS, V. Microbial genetic resources and Biodiscovery. In Ipek Kurtböke & Jean Swings (eds.), Microbial Genetic Resources and Biodiscovery WFCC Publications, UK.

NEIROTTI, E.; AZEVEDO, J. L. Técnica semiquantitativa de avaliação da produção de celulases em Humicola sp. Revista de Microbiologia; v. 19, p.78-81. 1988.

NOGUEIRA, E. B. S.; CAVALCANTI, M. A. Q. Cellulolytic fungi isolated from processed oats. Revista de Microbiologia, v. 24, p. 7-9, 1996.

PETZOLD, K.; SCHWIKAL, K.; HEINZE, T. Carboxymethyl xylan – Synthesis and detailed structure characterization. Carbohydrate Polymers, v. 64, p. 292–298, 2006.

POLIZELI, M. L. T. M.; RIZZATI, A. C. S.; MONTI, R.; TERENZI, H. F.; JORGE, J. A.; AMORIN, D. S. Xylanases from fungi: properties and industrial applications. Applied Microbiology and Biotechnology, v. 67, n. 5, p. 577-591, 2005.

RUEGGER, M. J. S.; TAUK-TORNISIELO, S. M. Atividade da celulase de fungos isolados do solo da Estação Ecológica de Juréia-Itatins, São Paulo, Brasil. Revista Brasil. Bot., v. 27, n. 2, p. 205-211, 2004.

SALES, M. R.; MOURA, R. B.; PORTO, T. S.; MACEDO, G. R.; PORTO, A. L. F. Variáveis que influenciam a produção de celulases e xilanase por espécies de Aspergillus. Pesq. agropec. bras. v.45, p.1290-1296, 2010.

SILVA, D. C. V.; TIAGO, P. V.; MATTOS, J. L. S.; PAIVA, L. M.; SOUZA-MOTA, C. M. Isolamento e seleção de fungos filamentosos do solo de sistemas agroflorestais do Município de Bom Jardim (PE) com base na capacidade de produção de enzimas hidrolíticas. Revista Brasil. Bot., v.34, p.607-610, 2011.

TEATHER, R. M.; WOOD, P. J. Use of congo red polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology. v. 43, p.777-780, 1982.




DOI: http://dx.doi.org/10.18067/jbfs.v2i4.75

Refbacks

  • There are currently no refbacks.
';



 

Follow us

FacebookTwitter

J. Bioen. Food Sci., Macapá, AP, Brazil. eISSN 2359-2710

  Licença Creative Commons 

The journal is licensed with Creative Commons Atribuição-NãoComercial-CompartilhaIgual 4.0 Internacional

 

Address for correspondence

Professor Victor Hugo Gomes Sales

Journal of Bioenergy and Food Science

Federal Institute of Amapá, Campus Macapá.

Rodovia BR 210 KM 3, s/n Bairro Brasil Novo. CEP: 68.909-398. Macapá, Brazil.

Phone: +55 (96) 3198-2150

jbfs@ifap.edu.br | suporte.jbfs@ifap.edu.br