Izothiazolone based biocides: efficiency evaluation on Staphylococcus warneri isolated from e-coat process rinse water

Daniel Ramos Oliveira Sutti, Leo Kunigk, Cynthia Jurkiewics

Resumo


The e-coat process is the first step in automotive painting and consists of immersing the part in a conductive water-based paint bath, followed by several rinse tanks and preceded by a rinse tank with demineralized water after pre-treatment. In this process, microbiological contamination is common, which is prevented or eliminated by adding isothiazolone based biocides combined with other active components. The objective of this study was to evaluate the efficiency of two commercial biocides containing the active components, 5-Chloro-2-methyl-2H-isothiazol-3-one (CIT) and 2-Methyl-2H-isothiazol-3-one (MIT), in the inactivation of two Staphylococcus warneri strains, isolated from contaminated water from tanks of an e-coat paint applicator company. The Minimum Inhibitory Concentration (MIC) was 5 mg/L and the Minimum Bactericide Concentration (MBC) was 10 mg/L for both biocides. Survival curves were obtained and the Weibull model was adjusted. The models obtained indicated lower resistance of S. warneri strain isolated from the demineralized water tank and higher inactivation rate of the biocide containing the association of isothiazolinone and ethylenedioxy (dimethanol).

Palavras-chave


Painting; Electrodeposition; Coatings. Weibull model; MIC

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Referências


BAPTISTA, A. B.; RAMOS, J. M. M.; NEVES, R. R.; SOUZA, D. F.;

PIMENTA, R. S. Diversidade de Bactérias Ambientais e de Pacientes no Hospital Geral de Palmas-TO. Journal of Bioenergy and Food Science, 2 (4), p. 160-164.

CUI, N,; et al. (2011). Toxicity profile of labile preservative bronopol in water: the role of more persistent and toxic transformation products. Enviromental pollution 159. p. 609-615.

DIEHL, M.A.; CHAPMAN, J.S. (1999). Association of the biocide 5-chloro-2-methyl-isothiazol-3-one with Pseudomonas aeruginosa e Pseudomonas fluorescens. International Biodeterioration & Biodegradation 44 p. 191-199.

HUANG, L. (2013) USDA Integrated Pathogen Modeling Program. (/Main/docs.htm?docid=23355). USDA Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA.

KLOOS, W. E.; SCHLEIFER, K. H.(1975). Isolation and Characterization of Staphylococci from Human Skin II. Descriptions of Four New Species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans. International Journal of Systematic Bacteriology. 25 (1): p.62–79.

LA ROSA, F. R.; et al.(2008) Contaminação microbiológica de tintas à base d´água de uma indústria do estado do Paraná, Brasil. Semina: Ciencias Exatas e da Terra, v.29, n.1, 85-92.

LEIGHTON P.M., LITTLE J.A. (1986) Identification of coagulase-negative Staphylococci isolated from urinary tract infections. Am. J. Clin. Pathol. 85(1):92-95.

MAFART, P; et al. (2002) On calculating sterility in thermal preservation methods: application of the Weibull frequency distribution model. International Journal of Food Microbiology 72 p.107 – 113.

MAIUTA, N.D., et al. (2009) Microbial degradation of formaldehyde in white mineral dispersins preserved with formaldehyde-releasing biocides. International Biodeterioration & Biodegradation 63 p. 769-777.

MORLEY, J.O., et al. (1998) Theoretical studies on the biocidal activity of 5-chloro-3 isothiazolone. Journal of Molecular Structure (Theochem) 429. p. 103-110.

ORR, C.P.(2008) Bacteria control in electrocoat DI & RO water systems. In: ECoat 2008 Conference, Indianapolis, Estados Unidos.

RAIKOS, V; et al. (2012) Water content, temperature and biocide effects on the growth kinetics of bacteria isolated from JP-8 aviation fuel storage tanks. Fuel 93, 559-566.

RIBEIRO, M.C.; STELATO, M.M. (2011) Microbiologia Prática: Aplicações de Aprendizagem de Microbiologia Básica. 2a. edição, São Paulo, Brasil.

ROSSMORE, H.W. (1990) The interaction of Formaldehyde, Isothiazolone and Copper. International Biodeterioration 26 (1990) 225-235.

SALFINGER, Y.; TORTORELLO, M. L. (2015) Compendium of Methods for the Microbiological Examination of Foods. 5.ed. Washington, DC: APHA, 995 p, 2015.

SUTTI, D.R.O. E-Coat / KTL (Texto de apoio didático). Treinamentos a clientes BASF, 2010

WANG, T. F.; LIN, T. J.; YANG, D. J.; ANTONELLI, J. A.; YASAD, H. K. Corrosion protection of cold-rolled steel by low temperature plasma interface engineering: I. Enhancement of E-coat adhesion. Progress in Organics Coatings, 28 (4). p. 291-297.

WEISBURG, W.G., BARNS, S.M., PELLETIER, D.A., LANE, (1991) D.J.16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology. 173 (2). p.697–703.

WILLIAMS, T.M. (2007) The mechanism of action of isothiazolone biocides. Power Plant Chemistry, 9 (1).




DOI: http://dx.doi.org/10.18067/jbfs.v7i2.278

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J. Bioen. Food Sci., Macapá, AP, Brazil. eISSN 2359-2710

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Journal of Bioenergy and Food Science

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