Volatile compounds detection with antibacterial activity in endophytic fungi associated with Costus spiralis / Detecção de compostos voláteis com atividade antibacteriana por fungos endofíticos associados à Costus spiralis

Deyze Alencar Soares, Poliana Guerino Marson Ascencio, Geovanka Marcelle Aguiar Leão, Katarina Mirna Tenório Marinho Rodrigues, Raphael Sanzio Pimenta

Abstract


- Endophytic fungi that live inside plants, for instance, in organs and plant tissues such as leaves and branches, without causing any harm. Assumptions of interactions between endophytic fungi and host plants suggest that therapeutic properties may be in the fungi and not on the plant or, probably, in the interaction amid them. Due to this, the interest in endophytic fungi from medicinal plants has increased. The gram-positive Streptococcus pneumoniae and gram-negative Klebsiella pneumoniae are important pathogenic bacteria, acting as infectious agents and causing respiratory diseases and hospital infections. This study aimed to obtain volatile compounds from endophytic fungi, which were isolated from medicinal plant Costus spiralis (jacq) Roscoe (Costaceae) and perform an antibacterial evaluation of them against S. pneumoniae and K. pneumoniae. The test to verify volatile substances was realized using Petri dishes without agar in the central portion, in order to prevent the diffusion effect. The endophytic fungus and the pathogen were inoculated on opposite sides of the plate that were sealed with parafilm. Negative controls were performed with each pathogen inoculation in culture media without antagonist, however, there was no growth inhibition of pathogens for those which were incubated in the presence of endophytic test, when comparing to control test. Thus, the discovery of new antibiotics becomes important due to the emergence of resistant pathogens, as well as the development of new diseases and the toxicity of drugs that already exist

Keywords


Klebsiella pneumoniae, Streptococcus pneumoniae, medicinal plant

References


ALBUQUERQUE, J.M. Plantas medicinais de uso popular. Associação Brasileira de Educação Agrícola Superior (ABEAS). Ministério da Agricultura – Secretaria Geral. Brasília. 1989.

ANTUNES, A.S.; SILVA, B.P; PARENTE, J.P. Flavonol glycosides from leaves of Costus spiralis. Fitoterapia, 71: 507-510, 2000.

BERDY, J. Bioactive microbial metabolites. A personal view. J Antibiot 58:1-26, 2005.

BRANDÃO, M. G. L.; COSENZA, G. P.; MOREIRA, R. A.; MONTE-MOR, R. L. M. Medicinal plants and other botanical products from the Brazilian Official Pharmacopoeia. Brazilian Journal of Pharmacognosy,v.16, n.3, p. 408-420, 2006.

CARTER, G. T. Natural products and Pharma: Strategic changes spur new opportunities. Natural product reports, 28(11), 1783-1789, 2011.

CRAGG, G. M.; NEWMAN, D. J. Biodiversity: a continuing source of novel drug led. Pure and Applied Chemistry. V. 77, n. 1, p 7-24, 2005.

CHARAUSIA, B.; PANDEY, A.; PALNI, L.M.S.; TRIVEDP, I.; KUMAR, B.; COLVIN, N. Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiological Research, Amsterdam, v.160, p. 75-81, 2005.

DEMAIN, A.L. Pharmaceutically active secondary metabolites of microorganisms. Applied Microbiology and Biotechnology. 52: 455-463, 1999.

DONADIO, S.; MAFFIOLI, S.; MONCIARDINI, P.; SOSIO, M.; JABES, D. Antibiotic discovery in the twenty-first century: current trends and future perspectives. The Journal of antibiotics, 63(8), 423-430, 2010.

HIGGINBOTHAM, S.J.; ARNOLD, A. E.; IBANEZ, A.; SPADAFORA, C.; COLEY, P.D.; KURSAR, T. A. Bioactivity of fungal endophytes as a function of endophytes taxonomy and the taxonomy and distribuition of their host plants. PloS One, San Francisco, v.8, n.9, p. 73192, 2013.

HYDE, K.D.; SOYTONG, K. The fungal endophyte dilemma. Fungal Diversity, 33: 163-173, 2008.

KUSARI, S.; LAMSHOFT, M.; ZUHLKE, S.; SPITELLER, M. An endophytic fungus from Hypericun perfolatum that produces hypericin. Journal of Natural Products 71: 159-162, 2008.

LIANG, Y. Production liquid transportation fuels from heterotropic microalgae. Applied Energy, v. 104, p. 860-868, 2013.

MARTINS, E.R.; CASTRO, D.M.; CASTELLANI, D.C.; DIAS, J.E. Plantas Medicinais. Editora UFV, Viçosa, 2003.

MEDEIROS, M.F.T.; FONSECA, V.S.; ANDREATA, R.H.P.; Plantas medicinais e seus usos pelos sitiantes da Reserva do Rio das Pedras, Mangaratiba, RJ, Brasil. Acta Botanica Brasilica. 18:391-399, 2004.

MAAS, P.J.M. Costoidae (Zingiberaceae). Flora Neotropica, Monograph, (8),1972. OBUTE, C. G.; ADUBOR, G. O. Chemicals Detected in Plants Used For Folk Medicine in South Eastern Nigeria. Ethnobotanical Leaflets, v.11, p.173-194, 2007. PIMENTA, R. S.; SILVA, J. F. M. ; BUYER, J. S. AND JANIEWICZ, W. J. Endophytic Fungi from Plums (Prunus domestica) and Their Antifungal Activity against Monilinia. Journal of Food Protection, Vol. 75, No. 10, p.1883–1889, 2012.

PORRAS-ALFARRO, A.; BAYMAN, P. Hidden fungi, emergent properties: endophytes and microbiomes. Annu Ver Phytopathol. Vol. 49, p. 291-315, 2011.

QUADRI, M.; JOHRI, S.; SHAH, B.A.; KHAJURIA, A.; SIDIQ, T.; LATTOO, S.K.; RIYAZ-UL-HASSAN, S. Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas. Springer Plus, 2 (1),8, 2013. SAS. Statistical Analysis System. SAS Institute Inc., Cary, NC, USA, 2001.

SILVA, B.P; PARENTE J.P. New steroidal saponins from rhizomes of Costus spiralis. Zeitschrift fur Naturforschung, Section C: Journal of Biosciences, 59:81-85, 2004.

SOLIMAN, S. S.; RAIZADA, M. N. Interactions between co-habitating fungi elicit synthesis of Taxol from an endophytic fungus in host Taxus plants. Frontiers in microbiology, 4, 2013.

STIERLE, A.; STROBEL, G.A.; STIERLE, D.; GROTHANS, P.; BIGNAMI, G. The search for a taxol producing microorganism among the endophytic fungi of the pacific yew, Taxus brevifolia. Journal of Nat. Products, 58: 1315-1324. 1995.

STROBEL, G.A. Rainforest endophytic and bioactive products. Critical Reviews in Biotecnology, 22: 315-333, 2002.

STROBEL, G.A.; DAISY, B. Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiol. and Mol. Biology Reviews, 67(04): 491-502, 2003.

VIEL, T.A.; DOMINGOS, D.C.; SILVA, A.P.M.; LIMALANDMAN, M.T.R.; LAPA, A.J.; SOUCCAR, C. Evaluation of the antiurolithiatic activity of the extract of Costus spiralis (Jacq.) Roscoe in rats. Journal of Ethnopharmacology, 66:193-198, 1999.

WORAPONG, J; STROBEL, G. A.; FORD, E. J.; LI, J. Y.; BAIRD, G; HESS, W. M. Muscodor albus anam. nov. na endophyte from Cinnamomum zeylanicum. Mycotaxon, v. 79, p. 67-79, 2011.




DOI: http://dx.doi.org/10.18067/jbfs.v2i4.60

Refbacks

  • There are currently no refbacks.
';



J. Bioen. Food Sci., Macapá, AP, Brazil. eISSN 2359-2710

  Licença Creative Commons 

The journal is licensed with Creative Commons Atribuição-NãoComercial-CompartilhaIgual 4.0 Internacional

 

Address for correspondence

jbfs@ifap.edu.br | suporte.jbfs@ifap.edu.br