Pretreatment of sugarcane top leaves by ozonation as a promotion of susceptibility to hydrolysis

Felipe Iwagaki Braga Ogando, Juliana Aparecida de Souza Sartori, Nathália Torres Corrêa Magri, Claudio Lima de Aguiar

Abstract


Studies about Ozone (O3) application in sugarcane residues for ethanol production are ongoing. This study was undertaken to evaluate the use of ozone as a pretreatment to facilitate hydrolysis of sugarcane top leaves. In the first experimentation, sugarcane top leaves were subjected to ozone (62.37 mg O3 min-1 over a three hour period) and water only (control). Following ozonation, the material was separated into a liquid and solid fraction. The analysis of liquid fraction showed that phenolic concentration and soluble solids did not vary. However, there was a decrease in pH and an increase in reducing sugars. This treatment increased saccharification. During the second treatment, ozone was added by direct infusion in a suspension of sugarcane top leaves and distilled water at a ratio of 1:10 (w.v-1).  Additionally, the lignocellulosic analysis showed a positive relationship between the dose of ozone and decomposition of lignin and an increase in the proportion of cellulose and hemicellulose in the material. All aspects are good signs for the use of ozone in pretreatment of lignocelullosic feedstocks.

References


(1) AGUIAR, C.L. Biodegradation of the cellulose from sugarcane sugarcane top leaves by fungal cellulase. Ciencia y Tecnologia Alimentaria, v.3, n.2, p.117-121, 2001. DOI:10.1080/11358120109487655

(2) RUBIN, E.M. Genomics of cellulosic biofuels. Nature, v.454, p.841-845, 2008. DOI:10.1038/nature07190

(3) SATHITSUKSANOH, N.; XU, B.; ZHAO, B.; ZHANG, Y.H.P. Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment. PLoS ONE, v.8, n.9, e73523, 2013. DOI:10.1371/journal.pone.0073523

(4) ESTEGHALIAN, A.; HASHIMOTO, A.G.; FENSKE, J.J.; PENNER, M.H. Modeling and optimization of the dilute-sulfuric-acid pretreatment of corn stover poplar and switchgrass. Bioresource Technology, v.59, n.2-3, p.129-136, 1997. DOI:10.1016/S0960-8524(97)81606-9

(5) CHUM, H.L.; JOHNSON, D.K.; BLACK S.; BAKER J.; GROHMANN K.; SARKANEN K.V.; WALLACE, K.; SCHROEDER, H.A. Organosolv pretreatment for enzymatic hydrolysis of poplars: I. enzyme hydrolysis of cellulosic residues. Biotechnology and Bioengineering, v.31, e.7, p.643-649, 1988. DOI:10.1002/bit.260310703

(6) THRING, R.W.; CHORENT, E.; OVEREND, R. Recovery of a solvolytic lignin: effects of spent liquor/acid volume ration, acid concentration and temperature. Biomass, v.23, e.4, p.289-30, 1990. DOI:10.1016/0144-4565(90)90038-L

(7) AZAM, A.M. Pretreatment of cane Sugarcane top leaves with alkaline hydrogen peroxide for enzymatic hydrolysis of cellulose and ethanol fermentation. Journal of Environmental Science and Health, Part B, v.24, n.4, p.421-433, 1989.

(8) VIDAL, P.F.; MOLINIER, J. Ozonolysis of lignin: improvement of invitro digestibility of poplar sawdust. Biomass, v.16, n.1, p.1-17, 1988. DOI:10.1016/0144-4565(88)90012-1

(9) GARCÍA-CUBERO, M.T.; COCA, M.; BOLADO, S.; GONZÁLEZ-BENITO, G. Chemical oxidation with ozone as pre-treatment of lignocellulosic materials for bioethanol production. Chemical Engineering Transactions, v.21, p.1273-1278, 2010. DOI:10.3303/CET1021213

(10) KOJIMA, Y.; YOON, S.L. Improved enzymatic hydrolysis of waste paper by ozone pretreatment. Journal of Material Cycles and Waste Management, v.10, n.2, p.134-139, 2008. DOI:10.1007/s10163-007-0198-5

(11) MACGILLIVRAY, A.W.; GRAHAM, W.S. Brix determination. Proceedings South Africa Sugar Technology Association, v.6, p.215-218, 1969.

(12) NELSON, N. A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry, v.153, e.2, p.375-380, 1944.

(13) PORTER, L.J.; HRSTICH, L.N.; CHAN, B.G. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry, v.25, n.1, p.223-230, 1985. DOI:10.1016/S0031-9422(00)94533-3

(14) PRIETO, P.; PINEDA, M.; AGUILAR, M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemestry, v.269, n.2, p.337-341, 1999. DOI:10.1006/abio.1999.4019

(15) TARGONSKI, Z. Alkali process for enhancing susceptibility of auto hydrolyzed beech sawdust to enzymatic hydrolysis. Enzyme and Microbial Technology, v.7, n.3, p.126-185, 1985. DOI:10.1016/0141-0229(85)90142-5

(16) FERRAZ, A.; MENDONÇA, R.; DA SILVA, F.T. Organosolv delignification of white- and brown-rotted Eucalyptus grandis hardwood. Journal of Chemical Technology and Biotechnology, v.75, n.1, p.18-24, 2000. DOI:10.1002/(SICI)1097-4660(200001)75:1<18::AID-JCTB169>3.0.CO;2-Z

(17) GOUVEIA, E.R.; NASCIMENTO, R.T.; SOUTO-MAIOR, A.M.; ROCHA, G.J.M. Validação de metodologia para a caracterização química de bagaço de cana-de-açúcar. Química Nova, v.32, n.6, p.1500-1504, 2009. DOI:10.1590/S0100-40422009000600026

(18) MADHO, S.; DAVIS, S.B. Review of proven technologies available for the reduction of raw sugar colour. Proceeding South Africa Sugar Technology Association, v.81, p.165-183, 2008.

(19) DÍAZ, M.F.; HERNÁNDEZ, R.; MARTÍNEZ, G.; VIDAL, G.; GÓMEZ, M.; FERNÁNDEZ, H.; GARCES, R. Comparative study of ozonized olive oil and ozonized sunflower oil. Journal Of The Brazilian Chemical Society, v.17, n.2, p.403-407, 2006. DOI:10.1590/S0103-50532006000200026

(20) ENAMI, S.; HOFFMANN, M.R.; COLUSSI, A.J. Prompt formation of organic acids in pulse ozonation of terpenes on aqueous surfaces. Journal of Physical Chemistry Letters, v.1, n.15, p.2374-2379, 2010. DOI: 10.1021/jz100847a

(21) BEN’KO, M.; MANISOVA, O.R.; LUNIN V.V. Effect of ozonation on the reactivity of lignocellulose substrates in enzymatic hydrolysis to sugars. Russian Journal of Physical Chemistry A, v.87, n.7, p.1108-1113, 2013. DOI: 10.1134/S0036024413070091

(22) JOHANSSON, E.E.; LIND, J.; LJUNGGREN, S. Aspects of the chemistry of cellulose degradation and the effect of ethylene glycol during ozone delignification of kraft pulps. Journal of Pulp and Paper Science, v.27, n.3, p.239-244, 2000.

(23) LIND, J.; MERENYI, G.; JOHANSSON, E.; BRINCK, T. The reaction of peroxyl radicals with ozone in water. Journal of Physical Chemistry A, v.107, n.5, p.676-681, 2003. DOI: 10.1021/jp026323u

(24) HEINZLE, E.; GEIGER, F.; FAHMY, M.; KUT, O.M. Integrated ozonation-biotreatment of pulp bleaching effluents containing chlorinated phenolic compounds. Biotechnology Progress, v.8, n.1, p.67-77, 1992. DOI: 10.1021/bp00013a010

(25) SOARES, S.E. Ácidos fenólicos como antioxidantes. Revista de Nutrição, v.15, p.71-81, 2002. DOI:10.1590/S1415-52732002000100008

(26) BOX, G.E.P.; HUNTER, W.G.; HUNTER, J.S. Statistics for experimenters: an introduction to design, data analysis and model building. 1 ed. New York: Wiley and Sons, 1978, 653 p.




DOI: http://dx.doi.org/10.18067/jbfs.v3i4.119

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